FastQCFastQC Report
Thu 26 Oct 2017
Arid1a_1_Arid1ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameArid1a_1_Arid1ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417017
Sequences flagged as poor quality0
Sequence length29
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCCAAAGAAAGAACTCTCATTTCTGA1068922.4200042698499917No Hit
TTCTTCACAGCGGTCGAGTGAATGATGTC844841.9126935667216134No Hit
TATTTGCAATTCACAGGGCAGCAGTAGTC524491.18743034043111No Hit
TTAACAGGGGGCAGCAGTAGTCAGTATGT491441.1126060868681285No Hit
CACCTGCACTAAATCCTGGCTGAGACTGG489271.1076932690093788No Hit
CTTCACTGACCTCAATCAGATTTTCAAAA463081.0483998589998635No Hit
TTAAAACGAAACAAAAGTTTATTGCTACT352320.7976423907809276No Hit
AATGCCAAAGCAAGAACTTTCATTTCAGA293880.6653359042992137No Hit
TCACAAACAACTATGGGATGATAAACATC279850.6335723860696031No Hit
AATGCCAAAGCAAGAACTTTCATTTCTGA219100.49603612573825273No Hit
TTTCCGTCGATAAAAACCTGTGGCGGATT217690.4928439261157474No Hit
ATGTTACTTTGAAATATTTTACTATAACT217550.4925269701248603No Hit
TGTGAGTTCGGCTTCTCTCCGGTGGCTTG208850.47283041926259284No Hit
CTTGGAAGGACCAGGGCAGCAGTAGTCAG208030.47097396274454006No Hit
CAGAAACGAGCCTCCCAGCAGACTCAAGA180290.4081713971216321No Hit
CATGCGCAGTTTATTAATAAGAGTATTCA166850.3777436219964741No Hit
GGGTATTCCTAGCTTCTAATGAACCGTAG161840.36640112546544423No Hit
ATACTAAATTAGACTTGATTTAAAACAAA160810.3640692349610608No Hit
ACCAACATTTAGAATGAACAAGACTAAAA155450.3519343484528133No Hit
GGGCAGCAGTAGTCAGTATGTCGTATGCC148700.3366525417493299No Hit
TTTAAAATAGCAAAGGCTTTAATGTGCGT145200.32872864197715335No Hit
TGAGTGATAATAAATCTGAAGGGAATCTG136530.30910001025579026No Hit
CATTACTTGGTGTTATACATTCACGCACT134740.3050475015151628No Hit
TCCCAGAGGAGCTGGAGGAAGTGTCTGGG132170.2992290951110218No Hit
TGCAGACGGACCTCACACTCTACAAGTCA128270.2903996067934536No Hit
TCAGTCTTCCTTTTGTAAGAAGGATAAGT116670.26413753897709696No Hit
TTTTGTATTTTATATTTATATATTTTTAT105530.23891689798794075No Hit
TGCTTGTCTTGTTCAGACGCTTCAAACAA96870.21931090598021247No Hit
ACTCTCTCTCTCTCTCTCTAAAGTGCTGT94160.21317554358518429No Hit
TGCTCGTCTTGTTCAGCTGCTTCAAACAA90880.2057497175129731No Hit
TGTCTTTTTTATTAAGCTCGACGAAAATC89550.20273863559954605No Hit
AGACACAAACAGCTTTGGATTTGATATCC87090.19716926604538768No Hit
ACATCTGCTCTGTGGTGGCCTCTTTGCCC86930.196807030627231No Hit
CAGACGCCTCAGATGAGAGCGAGTGTTAA86560.19596936122274378No Hit
CGGTATGGTCCAATGAACCTGGGTGCCAA81720.18501173982350533No Hit
CACTCGAGTGTGCATAAATAATGATACAA79610.1802347602465646No Hit
GCCTTCTTCCTCGTTATTTCCTCCTTCCT79470.17991780425567755No Hit
TACAGCTGTAATTACACACACACACACAC79150.17919333341936425No Hit
AAAGTCCTGCTGCAGAGACCAGACCGTTC77150.1746653906924062No Hit
ACCCCTGGACTGAAGTGTTTACATTTATG76640.1735107652970319No Hit
AGGGCAGCAGTAGTCAGTATGTCGTATGC74570.16882434457463036No Hit
ATTTCAGAGCAGACGCTGAATTAGAAAGG74150.16787347660196916No Hit
ATCATGGGCAGCAGTAGTCAGTATGTCGT73170.16565478466575972No Hit
TCAGGGGCAGCAGTAGTCAGTATGTCGTA73110.16551894638395098No Hit
ATGCTGTATAGGTCTTTCTCTGGTCTTTC72960.16517935067942913No Hit
ACGTTTATATGTTCTTCTTAATGTCAACT72190.1634360927295503No Hit
GGTGTATGATGTGCAGCACATCTTTAAAG71260.1613305993615148No Hit
TGGCATGGTGTAGATCTTTCCGTCGATAA70620.15988165768888823No Hit
TACAGCTGTAATTACACACACACACACGC68780.15571595038008681No Hit
AGCGCGTGACAGTAATGGTGACCTAATAA64690.14645630750345764No Hit
TGTAGACAACACTAGCCTCAGTCCTAGTG62510.1415208499310734No Hit
AGTCAGTTTAACTTGCTGGTCAGACACAT62270.14097749680383842No Hit
TGCAGACTGACCTCACACTCTACAAGTCA62240.14090957766293408No Hit
TTAATCTGTTACTGCTAATTTCTGGCTTT61630.13952855513121185No Hit
ACTGACTCGTCGTGTGACCACAAACGGCC61220.13860032687218546No Hit
ATGGTAAATGTGTATACACCAGGTGACGT59340.1343440607088449No Hit
CAGTTCATGTTTTGGCGCTGAAGCATATA58910.13337055302254894No Hit
AATTGAAAGTAATAATTATGGGCAGCAGT58500.13244232476352255No Hit
TCCAGGAACAGGATTAAGCACCCCTGCTA58360.13212536877263548No Hit
CATTAAATTCCAACCATGACAGAGCCGTG57410.1299745959773304No Hit
AACTGACCCATCCTCCTCCTTCCCTAAAC57110.1292954045682867No Hit
TATGTGGATTTGTAACAAAAATGTATAAT57100.1292727648546519No Hit
TCACTAGTGCTTGATTAAGGTACAGCGGC57020.12909164714557358No Hit
ACACGGTTTGCTTTTGTCCTGTCAAATGA56600.1281407791729124No Hit
TGGGCAGCAGTAGTCAGTATGTCGTATGC56230.12730310976842515No Hit
GCCGGGGCAGCAGTAGTCAGTATGTCGTA55960.12669183750028584No Hit
TTATTGCCACAGTATATTGTCATGATAAC55200.12497121926404178No Hit
TTCAAACGCAGTATAGAGCATGATGAGCT54480.12334115988233688No Hit
TAGAACCATGGCCTAGTTCTCAGTGTGAC54410.12318268188689335No Hit
TCAAACTTTGAAACGGTACAGCGGCCAGG53910.12205069620515384No Hit
ATAGGGCAGCAGTAGTCAGTATGTCGTAT53360.12080551195524038No Hit
ACTAGTAAGATGTGTCATTATTTGTAACT52750.11942448942351819No Hit
GGATGCGTAGGGCAGCAGTAGTCAGTATG51840.11736427548275227No Hit
ACCCCTGATTATACACACTGTTGGAAGCT51830.11734163576911749No Hit
AACACCTCTGATGATAAAACAAGCAAAAT51500.1165945252191694No Hit
AGTGAGTTCGGCTTCATTCCGGTGGCTTG51080.11564365724650821No Hit
CCAGGAGTACATTATTTTTAGTCAATACT50690.1147607084147514No Hit
TGCTTGTCTTGTTCAGGAGCTTCAAACAT50230.11371928158755105No Hit
ACGATTCTGGATTGTCTTTAAAACATCTG50170.11358344330574231No Hit
AGTGGTGCTGCATCAGCAAGCGTCTGCAT49990.11317592846031609No Hit
CTAATTTTTAGGAGGGAGTATTTTAAGCC49460.1119760236376722No Hit
TACTGGCCATCAACTGCTACTGAGACAGG49380.11179490592859388No Hit
ATGTCCAGTTCAGTGGACTGGTGTGCTGG49190.11136475136953289No Hit
TCACTCTAACAAGACAATCCGTCATGCCA48940.11079875852866311No Hit
CTCATAAGTACGGCGGCCAGGAAGGAGCT48720.11030068482869775No Hit
TCTGCCACACACTAATGAACTTAGTTAAA48390.10955357427874966No Hit
AGCTTCTTACAAAACCTCGTCTGGGTACG48100.10889702258334075No Hit
TTGGGGCAGCAGTAGTCAGTATGTCGTAT47420.107357522056175No Hit
AGTGTAGGGGCAGCAGTAGTCAGTATGTC46940.1062708158017051No Hit
ATGCTTATAAAGATGCTTATGCTTATGTT46820.1059991392380876No Hit
TTCGAGTCGACACTGTTTATCTGAAATGC46530.10534258754267868No Hit
GGTTATTCCTAGCTTCTAATGAACCGTAG46070.10430116071547835No Hit
AGCTGTCAATCACTCAAGACTTTCAGATG45870.10384836644278254No Hit
TCGCCAGACTTCCCTTTAAAGCATGCACG45770.10362196930643462No Hit
ATTTAGTGAGAGAATTAGATTCAAGATGT44460.10065616682027713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG250.001992704823.00240119
GGCGCTA950.023.00240119
ACTCCGA250.001992704823.00240117
GATCGGT352.2808388E-523.002417
GGGTCCG452.6601265E-723.002417
GATCCGA2400.023.002419
GTCCGGA1200.023.002416
CGCGTAC302.126181E-423.002416
CCGCCGG302.1263442E-423.0021415
GGGTACG6150.023.00162123
GGTACCA302.1266704E-423.0016223
CCCTAGC402.459692E-623.0008377
GGTACGT402.459934E-623.00057820
GGATCCT402.459934E-623.0005783
CGGATAC1350.023.0005782
CGCAACG703.6379788E-1223.0005781
GATCGCG1000.023.0005762
CGCGCAG502.8911018E-823.0005763
GCACGAC250.001993623623.0005769
GAACGCG302.1273231E-423.00057620
Produced by FastQC (version 0.11.5)